Search results for " GPC"

Evaluation and comparison of satellite precipitation estimates with reference to a local area in the Mediterranean Sea

2014

Precipitation measurement is a key activity for the analysis of storm processes as well as every hydrological process. Satellite retrieval systems, rain-gauge network and radar systems are complement to each other in terms of their coverage and capability of monitoring precipitation. Satellite rainfall estimates systems produce data with global coverage that can provide information in areas for which data from other sources are unavailable. Without referring to ground measurement, satellite-based estimates can be bias. Although some gauged adjusted satellite precipitation products are developed, an effective way of integrating multi-sources of precipitation information is still a challenge.…

Metabolite Sensing GPCRs: Promising Therapeutic Targets for Cancer Treatment?

2020

G-protein-coupled receptors constitute the most diverse and largest receptor family in the human genome, with approximately 800 different members identified. Given the well-known metabolic alterations in cancer development, we will focus specifically in the 19 G-protein-coupled receptors (GPCRs), which can be selectively activated by metabolites. These metabolite sensing GPCRs control crucial processes, such as cell proliferation, differentiation, migration, and survival after their activation. In the present review, we will describe the main functions of these metabolite sensing GPCRs and shed light on the benefits of their potential use as possible pharmacological targets for cancer treat…

Identification of GPR23/LPA4 as a candidate G protein-coupled receptor for Guanosine

2012

Guanosine-Induced Antiproliferative Effects Are Modulated by GPCR Expression in Human Glioma and Melanoma Cell Lines.

2012

Guanosine-Induced Antiproliferative Effects Are Modulated by GPCR Expression in Human Glioma and Melanoma Cell Lines.

Origin of neuronal-like receptors in Metazoa: cloning of a metabotropic glutamate/GABA-like receptor from the marine sponge Geodia cydonium.

1999

To date, no conclusive evidence has been presented for the existence of neuronal-like elements in Porifera (sponges). In the present study, isolated cells from the marine sponge Geodia cydonium are shown to react to the excitatory amino acid glutamate with an increase in the concentration of intracellular calcium [Ca2+]i. This effect can also be observed when the compounds L-quisqualic acid (L-QA) or L-(+)-2-amino-4-phosphonobutyric acid (L-AP-4) are used. The effect of L-QA and L-AP-4, both agonists for metabotropic glutamate receptors (mGluRs), can be abolished by the antagonist of group I mGluRs, (RS)-alpha-methyl-4-carboxyphenylglycine. These data suggest that sponge cells contain an mG…

Analisi della affidabilità dei database pluviometrici globali a scala locale. Applicazione al territorio siciliano

2012

Negli ultimi anni i sistemi di misurazione e di stima delle precipitazioni, sono stati arricchiti dalla possibilità di ottenere stime attraverso dati acquisiti da sensori satellitari. I prodotti di stima così ottenuti risultano comunque spesso fortemente distorti. Per tale ragione le stime sulla base di soli dati satellitari, vengono ulteriormente elaborate attraverso l'utilizzo di dataset che raccolgono dati di precipitazione provenienti da reti pluviografiche al fine di ridurre la distorsione che caratterizza i dati satellitari. È auspicabile che questi dataset siano quanto più precisi al fine di fornire dati adatti alla correzione dei dati satellitari e in particolare alla riduzione dell…

Recombinant expression, in vitro refolding, and biophysical characterization of the N-terminal domain of T1R3 taste receptor

2012

Facteur d'impact (5 ans) : 1,617Notoriété à 2 ans : Acceptable (biochem.res.methods); The sweet taste receptor is a heterodimeric receptor composed of the T1R2 and T1R3 subunits, while T1R1 and T1R3 assemble to form the umami taste receptor. T1R receptors belong to the family of class C G-protein coupled receptors (GPCRs). In addition to a transmembrane heptahelical domain, class C GPCRs have a large extracellular N-terminal domain (NTD), which is the primary ligand-binding site. The T1R2 and T1R1 subunits have been shown to be responsible for ligand binding, via their NTDs. However, little is known about the contribution of T1R3-NTD to receptor functions. To enable biophysical characteriza…

Interactions between cholinergic and fibroblast growth factor receptors in brain trophism and plasticity

2014

Acetylcholine, acting on both nicotinic receptors (nAChRs) and muscarinic receptors (mAChRs), plays a role in the regulation of synaptic plasticity, being involved in the regulation of cellular processes and cognitive functions, such as learning, memory and attention. Recently, G protein coupled receptors (GPCRs), including mAChRs, have been reported to transactivate tyrosine-kinase receptors (RTK), such as epidermal growth factor receptor (EGFR), and initiate their intracellular signaling. In this minireview we have first analysed the RTK transactivation mechanisms, involving cholinergic receptors, and thereafter the interplay between AChR and neurotrophic factor systems built up by FGF2 a…

Microstructure and rheological response of laboratory-aged SBS-modified bitumens

2019

Polymer additives are widely used to improve the performance of road bitumens including their resistance to hardening during oxidation, although their oxidative inhibitor effect has not been well documented. This study aims to investigate the effect of laboratory-simulated ageing on the microstructure, and rheological properties of Polymer Modified Bitumens (PMBs) prepared with a Styrene-Butadiene-Styrene (SBS) copolymer and sulphur as a cross-linker. The laboratory ageing was conducted through the Rolling Thin Film Oven (RTFO) and the Pressure Aging Vessel (PAV) procedures. The unaged and aged binders were characterised using the Multiple Stress Creep and Recovery (MSCR) test, Gel Permeati…

Pharmacological comparison of rat and human melanocortin 3 and 4 receptors in vitro.

2002

Abstract The melanocortin 3 and 4 receptors are G-protein-coupled receptors found in the hypothalamus with important role in regulation of the energy balance. In this study, we performed pharmacological comparison of the rat and human melancortin (MC) 3 and MC4 receptors. We transiently expressed the genes for these receptors individually in a mammalian cell line and determined the binding affinities to several MSH peptides. The results showed no major difference between the rat and human MC3 receptors while the rat MC4 receptor had higher affinity to several peptides compared with the human MC4 receptor. NDP-, α-, β-, γ-MSH, ACTH(1–24), HS014 and MTII had from 5- to 34-fold higher affinity…